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Human Embryonic Stem Cells for Treatment of Retinal Degeneration

In this webcast, Sandra describes how she and her colleagues have paved the path for bringing hESC-derived retinal pigmented epithelial (PRE) cells to the clinic for patients suffering from age-related macular degeneration. They have generated a robust and scalable, xeno-free, and defined protocol to derive hESC-RPE cells that are functional but also safe, pure, and immunocompatible. Recently they have derived a GMP clinical grade pluripotent stem cell line that will be differentiated to hESC-RPE and used in a clinical trial in the coming years.

Contact Sandra HERE

Link to Fredrik Lanner's research lab

Link to related ARTICLES


Sandra Petrus-Reurer
, Karolinska Institutet, Sweden 

                                                                      2020-04-28

Functional characterization of human PSC-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

In this video, Tanja presents her recently published work on the defined differentiation of human pluripotent stem cell-derived cortical neurons on laminin isoform 521. Compared to rat cortical cultures, the hPSC-derived networks showed unique patterns of bursting activity. The hPSC-derived networks developed synchronous activity, recapitulating the classical cortical activity. Moreover, principal component analysis reflects the species-specific and maturation state differences between the two hPSC-derived and rat networks. 

Contact Tanja HERE

Link to Susanna Narkilahti's research lab

Link to ARTICLE


Tanja Hyvärinen, Tampere University, Finland 

                                                                      2020-01-21

CELLULAR THERAPY FOR BURN/SKIN INJURIES: 
Laminin-based Epidermal Keratinocytes Culture System in Xeno-free and Chemically Defined Condition

The current expansion of autologous human keratinocytes to resurface severe wound defects still relies on murine feeder layer and calf serum in the cell culture system. Here, the authors report a completely xeno-free and defined culture system utilizing these laminins which enables robust expansion of adult human skin keratinocytes. The system is comparable to the 3T3-J2 co-culture system that may not only provide safer keratinocyte use in the clinics but also facilitate the broader use of other cultured human epithelial cells in regenerative medicine.

Contact Monica HERE

Link to ARTICLE


Monica Tjin, Duke-NUS Medical School, Singapore 

                                                                      2019-12-11

LAMININ 521 PROMOTES QUIESCENCE IN ISOLATED STELLATE CELLS FROM RAT LIVER

Hepatic stellate cells (HSC) are liver-resident mesenchymal stem cells, critical for liver regeneration. In this webcast, Friederike Rohn present evidence that coating culture plates with Biolaminin 521, an important element of their niche in the space of Disse, improve hepatic stellate cell (HSC) adhesion and better preserved their retinoid stores as well as quiescence- and stem cell-associated phenotype.

Contact Friederike HERE.


Friederike Rohn, Heinrich Heine University Düsseldorf, Germany

                                                                      2019-01-11

LAMININ 521 STABILIZES THE PLURIPOTENCY EXPRESSION PATTERN OF HUMAN EMBRYONIC STEM CELLS

Here, Dr. Halima Albalushi describes the short-term effects of Biolaminin 521 on the expression of different genes in feeder-cell-derived hES cell lines after being transferred to and cultured on Biolaminin 521.

Contact Halima HERE.
Link to Nordfertil Research Lab


Halima Albalushi, Karolinska Institutet and University Hospital, Sweden 
and Sultan Qaboos University, Oman

                                                                      2018-10-23

A SWITCH IN LAMB2 TO LAMB1 ISOFORMS DURING AGING CONTROLS ENDOTHELIAL CELL FUNCTIONS AND ENHANCES ENDOTHELIAL-TO-MESENCHYMAL TRANSITION

Here, Ph.D. student Julian Wagner decipher the role of the microenvironment underlying the impairment of endothelial cell functions by aging. Mimicking age-related modulation of laminin β1 versus β2 chain expression changes the functional properties and phenotype of endothelial cells.

Contact Julian HERE.


           Julian U.G. Wagner, Goethe University, Germany

                                                                      2018-05-31

MAKING BLOOD FROM HUMAN PLURIPOTENT STEM CELLS USING LAMININS AND HYPOXIC CONDITIONS

Dr. Roger Rönn presents his research on how to make blood from human pluripotent stem cells using laminin and hypoxic conditions. 

Contact Roger HERE.


                    Roger E. Rönn, University of Edinburg, UK

                                                                      2018-05-01