Biolaminin 421 LN (LN421)
Human recombinant laminin 421
Biolaminin 421 supports several tissue-specific cell types such as endothelial cells and kidney cells and has been suggested to be important for renal, synaptic, and inflammatory functions.
A biologically relevant culture environment
In the kidney, laminin 421 is produced by mesangial cells and is presumably important for the development and reparation of microvasculature and glomerulogenesis (Abrass, 2010) whereas kidney podocytes mainly bind to laminin 521 via the α3β1 integrin (Sachs and Sonnenberg, 2013). Laminin 421 has also been implicated in renal cell carcinomas (Lohi, 1996; Vainionpää, 2007) and glial brain tumors (Ljubimova, 2001).
In chemical synapses, the laminin 421 isoform is crucial for the correct localization of pre- and post-synaptic specializations. In mice lacking the laminin β2 chain, neuromuscular junctions fail to form active zones in the presynaptic terminal and thereby neurotransmitter release is disrupted (Patton, 2001). Laminin 421 has been shown to assist in the formation and stabilization of active zones by several mechanisms, including voltage-gated Ca2+-channels and integrins containing the α3 subunit (Carlsson, 2003; Cohen, 2000).
Recommended applications
Pancreatic cells
Biorelevant culture of pancreatic cells on Biolaminin substrates The basement membrane formed next to the beta-cells contains alpha-4 and alpha-5 laminin […]View application
Kidney cells
Biorelevant culture of renal cells on Biolaminin substrates Laminins play an important role in kidney development Both the tubular and […]View application
Neural cells
Biorelevant culture of neural cells on Biolaminin substrates Laminins are widely expressed in the nervous system and are essential for […]View application
Key features
Coating plates
1. Slowly thaw recombinant laminins at +4°C before use.
2. Dilute the thawed laminin stock solution with 1xDPBS containing Ca2+ and Mg2+.
3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 μg/cm2. The optimal coating concentration is cell line-dependent.
4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spread evenly across the surface. Note that the laminin matrix will be inactivated if let dry.
Important notes
- The laminin stock solution is long-term stable when stored at -20°C to -80°C. Please refer to the product-specific CoA for shelf life details.
- Repeated freeze-thawing should be avoided. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C to -80°C. Thawed, undiluted laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions.
- Avoid long exposure of the protein to ambient temperatures.
- For your convenience, the coated plates can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.
- The protocol can easily be made totally defined and animal origin-free with your choice of culture medium and enzyme.
- Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.
Product name
Biolaminin 421 LN
Product code
LN421-02
LN421-0501
Declaration
For research use only
Storage
-20°C to -80°C
Concentration
0.1 mg/ml
Appearance
Clear, colorless, buffered solution with a
pH of 7.2 with 10% glycerol and 0.02% NaN3
Shipping condition
Dry Ice
Protein name
Laminin 421 (Laminin-9)
Classification
Animal origin-free, human recombinant protein
Product application
Culture of endothelial cells, mesangial cells, and maturation of neuromuscular junctions
Size guide
Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose the culture well format and fill in the desired coating concentration to see the amount required.
Please see the coating instructions for concentration and volume guidelines.